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range coefficients for –Lon choice (Figure one—resource facts one) in comparison to monoculture progress charges calculated in a plate reader in ER2566 ∆folA/∆thyA (–Lon) as described in components and methods.
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to check this prediction, we reintroduced chromosomal Lon expression beneath the Charge of a constitutive promoter inside our assortment pressure, and repeated deep mutational scanning in Organic triplicate (Supplementary file 2). We consult with the two regimes as +Lon and –Lon selection. the standard of +Lon collection was comparable to that of –Lon assortment (Figure 2—determine dietary supplement one, Figure 2—figure nutritional supplement two). in step with our speculation, the distribution of variety coefficients shifted in direction of far more damaging values while in the +Lon assortment, depleting beneficial assortment coefficients and enriching for negative or null coefficients (Figure 2A).
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